FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831041
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16060861
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC213650.082.134224120
GAGCACA360700.049.006634120
AGAGCAC474150.037.2276118-119
AAGAGCA709250.024.857306118-119
GAAGAGC785100.022.560745116-117
GGAAGAG962600.018.422915120
CGGAAGA1086000.016.44127114-115
TCGGAAG1122900.015.7038555114-115
ATCGGAA1335300.013.433375112-113
GATCGGA1352900.013.139862112-113
AGATCGG1471350.012.14607110-111
CTAGATC93550.011.180996108-109
TAGATCG371600.011.17425112-113
CACGTCT59300.010.008659120
CACACGT50900.09.974545120
TTAGATC138100.09.901804116-117
GAGATCG489200.09.654602110-111
AAGATCG593600.09.587462116-117
CCGATCT92550.09.5692674
TCCGATC95600.09.2639723
Produced by FastQC (version 0.11.5)