FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14508211
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC139200.065.963036120
GAGCACA219550.042.82921120
AGAGCAC283900.032.626225118-119
AAGAGCA447500.021.046633118-119
GAAGAGC501750.018.544647118-119
GGAAGAG617450.016.124216120
TCGGAAG701000.014.202421120
CGGAAGA720700.013.491666118-119
ATCGGAA832350.012.672142120
GATCGGA848650.012.138265118-119
AGATCGG930250.011.164226118-119
TAGATCG230500.010.279102114-115
CTAGATC57750.09.273924112-113
GAGATCG377100.08.329056120
TTAGATC94250.07.8171377114-115
CAGATCG355900.07.710626120
AAGATCG332450.07.6929245116-117
CACGTCT55350.07.16667120
TGAGATC166900.07.0006404116-117
GCTAGAT27900.06.980373112-113
Produced by FastQC (version 0.11.5)