FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831048

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831048
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16612993
Sequences flagged as poor quality0
Sequence length62-126
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT86350.030.4368934
TCCGATC99800.027.357063
CTTCCGA117900.023.7675171
CGATCTA29050.022.3082855
TTCCGAT184700.015.3989882
AAAAAAA2654200.011.728869120
GCGTCGG79000.010.711877120
CCGTATC1015950.010.219915120
CGATCTC71350.010.1760675
TCTCGGG21800.09.911047120
GCCGTAT1106750.09.869506120
CGTATCA924900.09.843836120
GTATCAT942250.09.76449120
CGCCGTA1192100.09.374336120
GGTCGCC1479550.09.2162075120
GTCGCCG1405500.09.09114120
TGGTCGC1598500.09.048529120
TCGCCGT1334050.09.024691120
GTGGTCG1723500.08.733529120
CGATCTG92600.08.6832515
Produced by FastQC (version 0.11.5)