FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16612993
Sequences flagged as poor quality0
Sequence length50-126
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC227600.0130.42831120
GAGCACA398700.072.60378120
AGAGCAC557050.052.910767118-119
AAGAGCA861900.033.92879118-119
GAAGAGC942350.031.300526116-117
CCGATCT104800.030.7130684
TCCGATC115150.028.4979063
CTTCCGA118800.028.1023031
CGATCTA29850.026.826035
GGAAGAG1141900.025.620863116-117
CGGAAGA1329750.022.288025114-115
TCGGAAG1416550.020.627897114-115
TTCCGAT184450.018.2449072
ATCGGAA1644250.018.042213112-113
GATCGGA1696300.017.24276112-113
AGATCGG1827300.016.16393110-111
TAGATCG523150.014.619215110-111
GAGATCG555950.013.501364110-111
CAGATCG545050.013.364411110-111
CACGTCT37050.013.205731120
Produced by FastQC (version 0.11.5)