FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831051

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831051
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12790178
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC146250.076.3698120
GAGCACA232750.047.755924120
AGAGCAC319650.036.37467120
AAGAGCA481450.023.95657118-119
GAAGAGC551750.020.934645118-119
GGAAGAG685000.016.951452116-117
TCGGAAG799650.014.691953120
CGGAAGA827200.014.544671120
GATCGGA946200.013.20669120
ATCGGAA924700.013.193213120
AGATCGG1051750.011.780953118-119
TAGATCG240100.010.845094116-117
CTAGATC59150.010.622106116-117
CCTAGAT21350.09.465779120
TTAGATC96200.09.453479120
AAGATCG431700.09.432408118-119
GAGATCG342250.09.172241120
CACACGT41550.08.592862120
TAAGATC156700.08.511951120
CAGATCG331700.08.367289120
Produced by FastQC (version 0.11.5)