FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13888645
Sequences flagged as poor quality0
Sequence length50-126
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC171000.0100.48901120
GAGCACA290050.058.337948120
AGAGCAC401700.042.68217118-119
CCGATCT99100.028.7613284
AAGAGCA620850.027.547604118-119
TCCGATC114750.025.1708473
GAAGAGC688200.025.081207120
CTTCCGA119850.024.1087761
CGATCTA33050.023.9098835
GGAAGAG853350.020.259413118-119
CGGAAGA973050.017.70367114-115
TTCCGAT171700.017.3616772
TCGGAAG1011000.017.008554116-117
ATCGGAA1178650.014.688546112-113
CACACGT43300.014.275194120
GATCGGA1217700.014.17218114-115
AGATCGG1309450.013.15322110-111
TAGATCG407700.011.844871116-117
CACGTCT50850.011.09205120
GAGATCG414450.010.804384112-113
Produced by FastQC (version 0.11.5)