FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831057

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831057
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12261177
Sequences flagged as poor quality0
Sequence length50-126
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC168550.0103.822655120
GAGCACA277800.060.59523120
AGAGCAC385950.044.758774120
AAGAGCA591550.029.324419120
GAAGAGC651400.026.803377118-119
GGAAGAG789500.021.851332116-117
CGGAAGA913750.019.262018116-117
TCGGAAG950350.018.195686114-115
CCGATCT72550.017.6884674
TCCGATC82800.016.2802243
ATCGGAA1106850.015.898443114-115
ACACGTC37250.015.293162120
CTTCCGA91000.015.0558751
GATCGGA1158100.014.979304112-113
AGATCGG1243850.014.085368112-113
TAGATCG285450.012.860345116-117
CACACGT36750.012.117527118-119
CTAGATC57650.011.971867108-109
CGATCTA21400.011.3383385
GAGATCG491800.011.243973110-111
Produced by FastQC (version 0.11.5)