FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13470270
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC107900.043.96848120
GAGCACA144500.034.926563120
AGAGCAC192450.027.315763120
AAGAGCA324650.017.220066120
GAAGAGC342550.016.356297120
GGAAGAG433400.013.968148120
TCGGAAG471200.013.909509120
GATCGGA574900.012.485775120
CGGAAGA518700.012.314194120
TAGATCG158600.012.230047120
ATCGGAA593400.012.106924120
AGATCGG648700.011.170068120
CTAGATC43300.010.414277118-119
TTAGATC69350.08.729186118-119
AAGATCG318000.08.236456120
GTCTAGA8400.00348816187.353979120
GCTAGAT21600.07.149702120
GAGATCG270600.07.110897118-119
CAGATCG276400.06.503642120
CACGTCT37500.06.424436120
Produced by FastQC (version 0.11.5)