FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14564823
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC158500.082.751305120
GAGCACA262100.049.05648120
AGAGCAC360150.036.928135120
AAGAGCA554450.024.060713120
GAAGAGC630550.021.16965118-119
GGAAGAG799500.016.82208120
TCGGAAG945050.014.196462118-119
CGGAAGA966750.013.968087120
ATCGGAA1086700.012.471366114-115
GATCGGA1088150.012.407506116-117
AGATCGG1177400.011.479345112-113
CTAGATC71350.010.888381112-113
TAGATCG274200.010.813116114-115
AAGATCG495200.09.693843120
GAGATCG368850.09.401395120
TTAGATC119550.09.137367108-109
ACACGTC56500.09.02578120
CAGATCG331650.08.908952118-119
CACGTCT53900.08.7042675120
GCTAGAT28300.08.39138112-113
Produced by FastQC (version 0.11.5)