FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15408884
Sequences flagged as poor quality0
Sequence length50-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC170850.084.21295120
GAGCACA278050.054.581375120
AGAGCAC370100.039.919197118-119
AAGAGCA591300.025.155344118-119
GAAGAGC643850.022.885025116-117
GGAAGAG786600.018.882133116-117
CGGAAGA925750.016.094097114-115
TCGGAAG927450.015.941053114-115
ATCGGAA1092850.013.662722112-113
GATCGGA1090950.013.61431112-113
AGATCGG1194450.012.476356110-111
TAGATCG276050.011.323486110-111
CTAGATC59050.010.915012118-119
ACACGTC42050.010.228966120
GAGATCG485650.09.887079114-115
CAGATCG413450.09.708145110-111
CACACGT39500.09.255919120
AAGATCG349750.09.162795114-115
TTAGATC98750.08.883654114-115
AGAGATC193950.08.437002112-113
Produced by FastQC (version 0.11.5)