FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831067

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831067
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12394971
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC149200.071.80879120
GAGCACA228500.044.86557120
AGAGCAC301700.034.747536118-119
AAGAGCA466400.023.268652120
GAAGAGC527000.021.457281120
GGAAGAG649100.017.81765120
CGGAAGA769200.015.378949120
TCGGAAG770650.015.161564120
ATCGGAA919400.013.01013120
GATCGGA930900.012.672733118-119
AGATCGG1009550.011.678913118-119
CTAGATC60250.011.394734120
TAGATCG233050.011.301908120
AAGATCG430600.09.888117120
GAGATCG347000.09.645093120
TTAGATC94100.09.471415114-115
CAGATCG314000.08.451316120
TCTAGAT31900.08.071321114-115
CCTAGAT21450.08.001547120
CGCTAGA8354.2259308E-67.90572120
Produced by FastQC (version 0.11.5)