FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831075

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831075
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18442357
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC207100.072.59748120
GAGCACA314350.046.6621120
AGAGCAC433250.034.902744120
AAGAGCA696500.022.380947120
GAAGAGC763150.020.889343120
GGAAGAG967900.016.383032118-119
TCGGAAG1128150.014.455886120
CGGAAGA1154750.013.753362120
GATCGGA1346550.012.393486120
ATCGGAA1339950.012.325159120
AGATCGG1466300.011.990641120
TAGATCG284550.011.036145120
CTAGATC66800.010.779593120
CACACGT61600.09.849536120
ACACGTC62400.09.616411120
GAGATCG528050.09.330921120
AAGATCG612450.09.180932118-119
CACGTCT63000.08.995669120
TTAGATC114900.08.542298116-117
CAGATCG520500.08.505551120
Produced by FastQC (version 0.11.5)