FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14041895
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC164500.071.73519120
GAGCACA256850.046.266273120
AGAGCAC342200.035.63688120
AAGAGCA532350.022.972755120
GAAGAGC589900.020.719826120
GGAAGAG757350.016.431108120
TCGGAAG873600.014.688279120
CGGAAGA890200.014.391055120
GATCGGA1058550.012.4227120
ATCGGAA1045250.012.405598118-119
AGATCGG1143600.011.662223120
CACGTCT58750.011.309338120
TAGATCG262800.010.955741120
ACACGTC57350.010.620218118-119
CTAGATC59950.010.3328118-119
CACACGT56100.09.314687118-119
ACGTCTG78700.09.058083120
TTAGATC97200.09.007567114-115
GAGATCG449650.08.812215118-119
AAGATCG427450.08.808414118-119
Produced by FastQC (version 0.11.5)