FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13549354
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC121000.056.66091120
GAGCACA183800.039.224705120
AGAGCAC237850.029.740467118-119
GAAGAGC407250.018.229942120
AAGAGCA404000.017.923431120
GGAAGAG533100.013.932285118-119
TCGGAAG580650.013.286049120
CGGAAGA606600.012.644829120
GATCGGA703250.011.598234120
AGATCGG747550.010.851805120
ATCGGAA732600.010.780266120
TAGATCG221000.010.626488120
CTAGATC58850.010.62008120
TTAGATC100850.07.7934995118-119
CCTAGAT22800.07.7528353116-117
AAGATCG322750.07.383968118-119
GAGATCG285400.07.255331116-117
GCTAGAT25650.06.8914366120
CAGATCG265000.06.7895045120
ATAGATC131250.06.3010235120
Produced by FastQC (version 0.11.5)