FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20240391
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC235750.068.18425120
GAGCACA369900.046.258648120
AGAGCAC495900.035.126923120
AAGAGCA755050.023.181345118-119
GAAGAGC842350.020.734098120
GGAAGAG1014250.017.925085120
CGGAAGA1197450.015.533359120
TCGGAAG1239950.015.239054120
ATCGGAA1455050.013.06743120
GATCGGA1492050.012.743423118-119
AGATCGG1690050.011.397935120
TAGATCG415300.010.526555116-117
CTAGATC111350.09.722175120
CACACGT64150.08.795721120
AAGATCG669200.08.72577120
TTAGATC173550.08.5917015116-117
CAGATCG568500.08.367144120
GAGATCG625550.08.250681114-115
CACGTCT75700.07.4537053120
TAAGATC252000.07.263958120
Produced by FastQC (version 0.11.5)