FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14448900
Sequences flagged as poor quality0
Sequence length50-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC225100.0112.36966120
GAGCACA377350.065.89162120
AGAGCAC508250.049.465923118-119
AAGAGCA755000.032.80518118-119
GAAGAGC841650.029.683727116-117
GGAAGAG1026700.024.119812116-117
CGGAAGA1204250.020.895533114-115
TCGGAAG1250000.019.776045114-115
ATCGGAA1443050.017.596027112-113
GATCGGA1484100.016.769148112-113
ACACGTC71350.016.488914120
CACGTCT66750.016.309916120
AGATCGG1613050.015.757582110-111
CTAGATC98350.014.729684108-109
CACACGT68250.014.472256118-119
ACGTCTG87100.014.112085120
TAGATCG389400.013.866536110-111
CGTCTGA97100.013.743762120
GAGATCG555250.013.337836110-111
AAGATCG513150.012.712569110-111
Produced by FastQC (version 0.11.5)