FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831084

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831084
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16594712
Sequences flagged as poor quality0
Sequence length56-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG101400.012.070853120
TGGTCGC459350.09.221609120
GTGGTCG507000.09.13598120
GGTGGTC549150.08.391044120
GGTCGCC446750.08.259549120
CTCGGTG686950.08.183898120
AGATCTC862250.07.9674253120
TCGGTGG648300.07.940668120
AAAAAAA796400.07.9029636120
TAGATCT897550.07.87467120
CGCCGTA389750.07.804904120
GATCTCG837950.07.790343120
GTCGCCG467650.07.7492876120
ATCTCGG779900.07.7085824120
CGGTGGT627000.07.664975120
CGTATCA326900.07.5434957120
TCTCGGT720000.07.5249047120
GCCGTAT385400.07.3636847120
GTGTAGA1140100.07.341368120
CCGTATC393400.07.10718120