FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831086

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831086
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12091149
Sequences flagged as poor quality0
Sequence length53-126
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG18900.023.17279120
CGGTGGG17150.017.84117120
AAAAAAA2716200.015.488075120
GCGTCGG62400.013.4604845120
CATTAAA914650.011.10502120
GTATCAT1199500.010.363507120
TCATTAA969200.010.356185120
CCGTATC1378900.010.320463120
GCCGTAT1500800.09.993887120
ATCATTA1072550.09.833737120
CGTATCA1258000.09.709892120
TATCATT1212850.09.680572120
CGCCGTA1619300.09.458903120
CTAGGTG6101.4260877E-99.343532114-115
GTCGCCG1905950.09.260798120
GGTCGCC2032050.08.942979120
TCGCCGT1769700.08.916069120
GGTCCCG7451.4599634E-48.8622881
TGGTCGC2193050.08.806224120
GTGGTCG2360950.08.614503120
Produced by FastQC (version 0.11.5)