FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12091149
Sequences flagged as poor quality0
Sequence length50-126
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC199750.0174.20352120
GAGCACA340800.0102.76182120
AGAGCAC462100.075.587456118-119
AAGAGCA684100.049.892597118-119
GAAGAGC747800.046.231995116-117
GGAAGAG906650.037.274185116-117
CGGAAGA1061550.032.561344114-115
TCGGAAG1102550.030.615305114-115
CACGTCT45200.030.035757120
ACACGTC44250.028.297632120
ATCGGAA1280550.027.305838112-113
CACACGT41500.026.837893118-119
GATCGGA1320950.025.921946112-113
AGATCGG1411550.024.738733110-111
ACGTCTG57500.022.693798120
TAGATCG330600.021.846783110-111
CTAGATC81650.020.338745110-111
CAGATCG373300.020.265797110-111
CGTCTGA70950.020.063705120
GAGATCG428500.020.054258110-111
Produced by FastQC (version 0.11.5)