FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831088

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831088
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15236329
Sequences flagged as poor quality0
Sequence length52-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCT158000.010.176572120
AGAGTGT270100.09.9290285120
TAGGGAA453600.09.880327120
AGTGTAG221900.09.760536120
GAGTGTA236650.09.709929120
GTAGGGA474300.09.385872120
TGTAGGG519000.09.06301120
GTGTAGG575050.08.607403120
TCGTGTA652900.08.527566120
GTGTAGA233600.08.270025120
AAGAGTG313900.08.256885120
CGTGTAG607300.08.249107120
GTAGATC247750.08.20937120
GTCGTGT692900.07.99199120
CGTCGTG777000.07.9531646120
TGTAGAT291250.07.7866316120
AGCGTCG876200.07.5388985120
AGGGAAA533250.07.4594436120
GAGCGTC938750.07.4264283120
GGGAAAG481300.07.279823120
Produced by FastQC (version 0.11.5)