FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831089

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831089
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15236329
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC130000.053.10208120
GAGCACA190050.038.589554120
AGAGCAC248950.029.860657120
AAGAGCA407100.018.858355120
GAAGAGC438650.018.128048120
GGAAGAG550450.014.740942120
TCGGAAG608900.013.817952120
CGGAAGA640500.013.058263120
ATCGGAA739850.012.106203120
GATCGGA737900.011.85906120
AGATCGG829450.011.739082120
TAGATCG199750.09.967899120
CTAGATC49950.09.371854120
AAGATCG362050.08.60264120
TTAGATC89300.07.7234364118-119
GAGATCG326250.07.6717353120
CACACGT36100.07.0888605120
CAGATCG346550.06.9521823118-119
CCTAGAT25000.06.740988120
TAAGATC148300.06.523645118-119
Produced by FastQC (version 0.11.5)