FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12278770
Sequences flagged as poor quality0
Sequence length50-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC167350.073.44152120
GAGCACA268100.044.80381120
AGAGCAC351650.035.56464120
AAGAGCA515600.024.87708120
GAAGAGC593300.021.505426118-119
GGAAGAG725900.017.478102120
CGGAAGA853150.015.191329116-117
TCGGAAG866650.014.582225116-117
ATCGGAA1001050.013.0093114-115
GATCGGA1042700.012.835615120
CTAGATC57000.012.338695120
TAGATCG222350.011.900608120
CACGTCT54300.011.633715118-119
AGATCGG1149250.011.497785112-113
ACGTCTG70350.010.987062120
TTAGATC79050.010.362319118-119
CCTAGAT20450.010.35341118-119
TCTAGAT24500.09.916942112-113
ACACGTC60350.09.719777118-119
CACACGT59000.09.677976120
Produced by FastQC (version 0.11.5)