FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831103

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831103
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14706592
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC196350.083.69448120
GAGCACA306250.052.08534120
AGAGCAC423000.038.662487120
AAGAGCA627300.026.334751120
GAAGAGC724300.023.185585120
GGAAGAG898350.018.74607118-119
CGGAAGA1074400.015.704465118-119
TCGGAAG1074350.015.664642120
ATCGGAA1246800.013.642597116-117
GATCGGA1267800.013.2606535114-115
ACACGTC57750.012.215069120
AGATCGG1397050.012.190744114-115
TAGATCG321450.011.487505120
CACGTCT55800.011.222945120
CTAGATC78700.011.050813118-119
CACACGT51300.010.928506118-119
GAGATCG525400.09.7406025112-113
AAGATCG494150.09.6577635120
TTAGATC107100.09.563368114-115
CCTAGAT24550.09.382537120
Produced by FastQC (version 0.11.5)