FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18546599
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC187100.063.815823120
GAGCACA289150.043.438046120
AGAGCAC386250.032.805412120
AAGAGCA620750.021.180256120
GAAGAGC682300.019.135643120
GGAAGAG865500.015.590565120
TCGGAAG981400.013.871293118-119
CGGAAGA1033450.013.492743120
GATCGGA1168600.012.250709120
ATCGGAA1167750.012.15341120
AGATCGG1282750.011.19616120
TAGATCG327200.010.374771120
CTAGATC69500.09.608837112-113
CACGTCT72100.09.054264120
ACACGTC75300.08.669488120
GAGATCG506400.08.50822120
CACACGT67650.08.474917118-119
CAGATCG502600.08.04001120
AAGATCG398700.07.9247866120
TTAGATC93350.07.8865085118-119
Produced by FastQC (version 0.11.5)