FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831109

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831109
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14241041
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC162000.079.31411120
GAGCACA258600.050.447506120
AGAGCAC352150.036.98673118-119
AAGAGCA534300.024.479086118-119
GAAGAGC614250.021.790972120
GGAAGAG768050.017.886932120
CGGAAGA934550.014.750476118-119
TCGGAAG935750.014.659026118-119
ATCGGAA1085750.013.221945120
GATCGGA1098550.012.752299118-119
AGATCGG1207050.011.673925120
TAGATCG292450.010.641096118-119
CTAGATC66400.010.528073114-115
GAGATCG413800.09.627827116-117
AAGATCG473650.09.458042120
TTAGATC117850.09.128099116-117
GTCTAGA9454.958565E-88.619147120
CAGATCG366300.08.6165120
ACACGTC54650.08.56986120
TGAGATC158700.08.531917116-117
Produced by FastQC (version 0.11.5)