FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12113863
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC152000.090.082405120
GAGCACA239550.054.02197120
AGAGCAC325000.041.15103118-119
AAGAGCA490800.027.978136120
GAAGAGC559450.023.87161116-117
GGAAGAG685950.019.537079118-119
CGGAAGA806050.016.767536114-115
TCGGAAG805000.016.677473116-117
ATCGGAA936350.014.584567112-113
GATCGGA942750.014.290591114-115
AGATCGG1023850.013.352821110-111
GCCTAGA6402.1179867E-512.232459120
CACGTCT46100.012.215171118-119
TAGATCG225650.012.045006112-113
CTAGATC39750.011.707586112-113
ACACGTC53050.010.772865120
GAGATCG417400.010.652843112-113
CACACGT46700.010.5613120
AAGATCG326350.010.101408110-111
CAGATCG343900.09.950235112-113
Produced by FastQC (version 0.11.5)