FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13106627
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC147750.056.36255120
GAGCACA225000.038.697647120
AGAGCAC284000.030.014593118-119
AAGAGCA455600.020.226038120
GAAGAGC490500.018.039637120
GGAAGAG591300.015.812657120
TCGGAAG681000.014.7024555120
CGGAAGA688150.013.886221120
GATCGGA817650.012.638536120
ATCGGAA829550.012.340957120
CACACGT47450.011.519433120
AGATCGG915300.011.318872118-119
TAGATCG224550.010.983152116-117
CTAGATC60000.010.182327116-117
TTAGATC81800.09.709229118-119
CCTAGAT20100.09.27834114-115
GAGATCG387100.08.83765120
AAGATCG283100.08.449897120
CACGTCT48750.08.4421625120
GCTAGAT24800.08.297488120
Produced by FastQC (version 0.11.5)