FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12839085
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC129550.068.00128120
GAGCACA194450.043.897846120
AGAGCAC263750.033.502415120
AAGAGCA401750.022.177162120
GAAGAGC463550.019.292513118-119
GGAAGAG569650.016.812195120
TCGGAAG659350.014.292207118-119
CGGAAGA679250.013.93244120
GATCGGA769850.012.258148116-117
ATCGGAA777250.011.9396305118-119
AGATCGG844450.010.9302435116-117
CTAGATC48250.010.858175114-115
TAGATCG203400.010.401355116-117
TTAGATC86100.09.069102116-117
AAGATCG349250.08.503704114-115
GAGATCG310800.07.9881544114-115
CCTAGAT14850.07.864953114-115
CAGATCG310350.07.2793303114-115
GCTAGAT20750.07.0759254108-109
ATAGATC98300.06.6536055112-113
Produced by FastQC (version 0.11.5)