FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831124

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831124
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13437451
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA1294800.010.912772120
TCCGATC64850.09.6217063
CGTATCA459150.09.384218120
CCGTATC523850.09.063787120
GCCGTAT557700.08.966853120
GGTCGCC713000.08.954998120
CGCCGTA591750.08.705128120
GGTGGTC875050.08.651421120
GTATCAT454700.08.629036120
GTCGCCG702150.08.621994120
GTGGTCG806700.08.541479120
TGGTCGC747400.08.502575120
CCGATCT70000.08.4852744
CGGTGGT962250.08.305194120
GCGTCGG77000.08.206127120
TCATTAA403550.08.171897120
TCGGTGG1019250.08.106427120
CTCGGTG1094150.08.030003120
TCGCCGT665300.07.8422594120
TCTCGGG31950.07.722407120
Produced by FastQC (version 0.11.5)