FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13437451
Sequences flagged as poor quality0
Sequence length50-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC185650.087.17535120
GAGCACA306800.052.775795120
AGAGCAC402300.040.4039118-119
AAGAGCA609550.027.290142120
GAAGAGC684500.023.588024116-117
GGAAGAG825800.019.40246116-117
CGGAAGA966900.016.826408114-115
TCGGAAG986900.016.136675114-115
ATCGGAA1159950.014.195739112-113
CACGTCT57400.014.130361120
GATCGGA1177800.013.707402112-113
CACACGT52600.013.563732120
ACACGTC61950.012.971311120
AGATCGG1295800.012.724801112-113
TAGATCG344100.011.928757110-111
ACGTCTG71000.011.529474120
CTAGATC85850.011.288407110-111
CGTCTGA82650.010.176924120
GAGATCG432200.010.129181110-111
CAGATCG481550.010.116156112-113
Produced by FastQC (version 0.11.5)