FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831126

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831126
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16553356
Sequences flagged as poor quality0
Sequence length52-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA1915900.013.664136120
GCGTCGG117500.013.430361120
GTCTAGG6502.1001324E-712.000513120
TCTCGGG40550.010.949913120
CGTATCA868350.09.597922120
GTATCAT838800.09.399544120
CTAGGCG5150.00526712289.3251251
ATCATTA770850.09.146139120
CCGTATC976450.09.088411120
TCATTAA679750.08.98604120
GCCGTAT1049350.08.977369120
GGTCGCC1353200.08.854944120
CGCCGTA1107450.08.679769120
TATCATT883600.08.569855120
TGGTCGC1429450.08.500135120
CATTAAA664750.08.295201120
GTGGTCG1540700.08.264129120
TCGCCGT1238350.08.101448120
GTCGCCG1310800.08.088535120
GGTGGTC1649700.07.852672120
Produced by FastQC (version 0.11.5)