FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16553356
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC250750.0100.78393120
GAGCACA408400.060.06835120
AGAGCAC553500.045.071262118-119
AAGAGCA810650.030.403963118-119
GAAGAGC918400.027.119434116-117
GGAAGAG1120900.021.992785116-117
CGGAAGA1307150.019.143532114-115
TCGGAAG1342100.018.219381114-115
ATCGGAA1557750.016.213097112-113
GATCGGA1614850.015.301311112-113
AGATCGG1756550.014.382435110-111
CACGTCT75250.013.651823120
TAGATCG410450.012.886888110-111
CACACGT69300.012.748595118-119
ACACGTC79200.011.933279120
CTAGATC68450.011.767962108-109
GAGATCG726750.011.762728110-111
CAGATCG612450.010.998617110-111
TTAGATC119100.010.904204108-109
AAGATCG423750.010.639633110-111
Produced by FastQC (version 0.11.5)