FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11680849
Sequences flagged as poor quality0
Sequence length52-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG42750.010.108104120
TAGATCT353150.09.075182120
GTGGTCG188650.08.589745120
GGTCGCC166500.08.506895120
TGGTCGC173050.08.3583145120
ATCTCGG311400.08.114033120
GTCGCCG166400.08.079193120
TCGGTGG261300.08.038999120
GATCTCG332250.08.038376120
CGGTGGT258350.07.991407120
GTAGATC459800.07.883903120
TCTCGGT291900.07.8130894120
AGTGTAG477450.07.693019120
AGATCTC351000.07.677371120
CTCGGTG279100.07.633818120
GTGTAGA467200.07.6177225120
GGTGGTC215200.07.613663120
AGAGTGT556100.07.533136120
GAGTGTA512850.07.4194584120
TAGGGAA815150.07.1786103120
Produced by FastQC (version 0.11.5)