FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831129
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11680849
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC122700.062.254917120
GAGCACA195350.040.069256120
AGAGCAC254050.033.374287120
AAGAGCA412550.021.420197120
GAAGAGC425550.020.444727118-119
GGAAGAG542150.016.071516120
TCGGAAG619400.015.234121120
CGGAAGA619800.014.178935116-117
GATCGGA746700.012.663118120
TTAGATC85500.012.338692120
ATCGGAA750600.012.19838118-119
AGATCGG806900.011.512708118-119
TAGATCG193150.010.738414118-119
CTAGATC52300.09.712147116-117
AAGATCG385800.09.401848120
GAGATCG257700.08.377116118-119
CAGATCG235400.07.953416116-117
CCTAGAT19150.07.4812493120
TCTAGAT31150.07.1087623114-115
GTCTAGA10200.00176804167.0228395120
Produced by FastQC (version 0.11.5)