FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12529141
Sequences flagged as poor quality0
Sequence length56-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG47900.013.778303120
TAGATCT481650.08.906623120
AGATCTC470500.08.582108120
GGTCGCC241400.08.549873120
GGTGGTC302350.08.413843120
TCGGTGG345700.08.400101120
ATCTCGG419400.08.397465120
GTGGTCG263150.08.025606120
TCTCGGT392250.08.015061120
GATCTCG447250.07.968464120
AGTGTAG643900.07.817755120
TGGTCGC247050.07.7957644120
CGCCGTA216350.07.570844120
CTCGGTG365900.07.5592213120
GTAGATC608600.07.5121036120
GTGTAGA628650.07.45385120
GAGTGTA694450.07.4214835120
GTCGCCG241250.07.2619634120
AAGAGTG820750.07.076247120
TGTAGAT660800.07.0276394120
Produced by FastQC (version 0.11.5)