FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831134

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831134
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10224418
Sequences flagged as poor quality0
Sequence length62-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGTA349150.09.280955120
AGATCTC234400.08.909075120
TAGATCT233850.08.904367120
GTGTAGA326050.08.834216120
AGTGTAG319800.08.320667118-119
AGAGTGT382600.08.202905120
TCTCGGT203050.08.038534120
GTAGATC339400.08.027032120
AAGAGTG425100.08.003923120
TGGTCGC135500.07.927289120
ATCTCGG220700.07.8579120
GCGTCGG87150.07.711904120
GTGTAGG738450.07.679301120
GTGGTCG152850.07.6556163120
TGTAGAT345700.07.5335784120
TGTAGGG676450.07.478299120
GGGAAAG593100.07.39606120
GATCTCG258250.07.3427324120
TAGGGAA604100.07.2751474118-119
GTAGGGA634200.07.1154532120
Produced by FastQC (version 0.11.5)