FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14652890
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC190250.085.44343120
GAGCACA308700.053.885044120
AGAGCAC422800.039.07282120
AAGAGCA629250.026.532122118-119
GAAGAGC725050.022.695927120
GGAAGAG876050.019.086065116-117
CGGAAGA1051750.016.141943120
TCGGAAG1056250.015.816829114-115
ATCGGAA1228100.013.57987112-113
GATCGGA1271250.013.175137112-113
CACGTCT50750.011.967537120
AGATCGG1393750.011.939802110-111
CTAGATC91150.011.523452120
TAGATCG341900.011.233652114-115
CACACGT46750.010.240356120
TTAGATC135000.09.871304116-117
AAGATCG527200.09.575583112-113
GAGATCG480550.09.12258114-115
GCCTAGA12006.481823E-78.931655120
CAGATCG452450.08.907114112-113
Produced by FastQC (version 0.11.5)