FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831140

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831140
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12817319
Sequences flagged as poor quality0
Sequence length57-126
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT27850.017.880734
AAAAAAA9171400.016.11583120
TCCGATC36150.014.9371113
CGGTGGG9750.014.768704120
TGTCAGG22000.013.0948871
TCTCGGG18300.011.802858120
CGTCAGG19050.011.3420281
CGATCTA9552.0372681E-1011.3084095
GGGGTGA7451.0087024E-711.2746782
GTCGGAC15600.011.1533612
GTACAGG19400.011.1335372
GGGACCA4350.001304345811.0340032
TGGGCCG5608.162867E-510.7175411
GATAGGG7309.43337E-710.6844672
TGTAACG31650.010.6193391
TGTTCAG68350.010.5372171
GTCAGGA31650.010.4260872
TGGTCCG9904.3964974E-910.3061621
CGTACGG8859.794894E-810.1725811
GTCCAGC23200.010.0857692
Produced by FastQC (version 0.11.5)