FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831142

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831142
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11024314
Sequences flagged as poor quality0
Sequence length57-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCAC65600.016.0120831
CCTTGAT100400.015.6034411
CCTTCAT83600.015.2210021
CCTCCAT53350.014.4008851
AAAAAAA1774750.013.5801120
CCTTTAC68750.013.2704151
CCTTTAT98700.012.6491051
CATTCAT91550.012.5879941
CCTTACC36400.012.5321581
CCTCGAT57950.012.3255741
CCTGCAC62050.012.1882791
CATTACC50850.012.1579381
CCTGCAT68250.012.0484591
CCTTACT27500.011.7862231
CGTATCA766300.011.7600565120
CATGCAT28100.011.7481641
CATTAAA556150.011.554091120
CCCTCAT28700.011.5025581
CAATACC58050.011.47715951
CATTACG37000.011.3555971
Produced by FastQC (version 0.11.5)