FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15862561
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC187650.081.750015120
GAGCACA295550.050.11803120
AGAGCAC404250.038.017445120
AAGAGCA615200.025.3247120
GAAGAGC703900.022.044657118-119
GGAAGAG871600.017.916016118-119
CGGAAGA1044050.015.057906116-117
TCGGAAG1051050.015.007757116-117
ATCGGAA1227300.012.958133114-115
GATCGGA1254350.012.841521120
AGATCGG1364500.011.655312118-119
CACGTCT64650.011.542943120
TAGATCG350050.010.708465116-117
TTAGATC121300.010.136783116-117
CTAGATC79300.09.879127118-119
ACACGTC68750.09.728147120
CACACGT64200.09.3572445118-119
GAGATCG569750.09.328845112-113
CCTAGAT26900.08.488566112-113
CAGATCG479950.08.453942112-113
Produced by FastQC (version 0.11.5)