FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11928948
Sequences flagged as poor quality0
Sequence length50-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC149200.093.47253120
GAGCACA243250.057.30125120
AGAGCAC333550.042.00491118-119
AAGAGCA500800.028.845444120
GAAGAGC595000.023.960535120
GGAAGAG710600.020.430647118-119
CGGAAGA833750.017.245163116-117
TCGGAAG844100.017.074076116-117
ATCGGAA967450.014.838432114-115
GATCGGA1014250.014.191075114-115
AGATCGG1106950.012.98282112-113
TAGATCG218550.012.124962120
CTAGATC53300.010.725017116-117
AAGATCG395900.09.859252112-113
TTAGATC78550.09.735391114-115
GAGATCG440550.09.66781112-113
CAGATCG436800.09.074754112-113
CACGTCT35450.08.542931120
TAAGATC140550.08.376841110-111
GCTAGAT25050.08.160545120
Produced by FastQC (version 0.11.5)