FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8221296
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC90300.063.824017120
GAGCACA146050.043.610367120
AGAGCAC190350.032.648148120
AAGAGCA294650.021.79187118-119
GAAGAGC324600.019.04603120
GGAAGAG394700.016.178751116-117
TCGGAAG460500.014.139241120
CGGAAGA447250.013.924142118-119
ATCGGAA542750.013.314968120
GATCGGA562550.012.456695120
AGATCGG631900.011.752723120
TAGATCG168350.011.37307120
TTAGATC65100.010.100748120
CACACGT27950.09.91793120
GAGATCG275450.09.689289120
CTAGATC44800.09.209506120
AAGATCG219350.08.405485120
CAGATCG206150.08.318264120
ACGTCTG33950.08.165129120
CACGTCT31850.07.590379118-119
Produced by FastQC (version 0.11.5)