FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13695191
Sequences flagged as poor quality0
Sequence length77-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCGC266450.09.315048120
GTGGTCG293350.08.973024120
TAGATCT527150.08.937841120
GGTGGTC322600.08.606537120
ATCTCGG471850.08.558865120
GATCTCG513600.08.518383120
TCGGTGG378050.08.456939120
TCTCGGT431400.08.455891120
CTCGGTG413100.08.452238120
AGTGTAG713150.08.426941120
AGATCTC511300.08.155438118-119
GGTCGCC274900.08.110544120
GTGTAGA686650.08.069494120
GAGTGTA764250.07.8163095120
CGGTGGT372500.07.6956077120
GTAGATC680150.07.651807120
AGAGTGT822150.07.5728526120
AAGAGTG897200.07.4618545120
TGTAGAT705400.07.2075176120
CGTATCA188700.06.9746594120
Produced by FastQC (version 0.11.5)