FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13695191
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC147200.072.30041120
GAGCACA232750.045.925842120
AGAGCAC308250.034.75067118-119
AAGAGCA479000.022.246222120
GAAGAGC531250.020.143785116-117
GGAAGAG644100.016.988539120
CGGAAGA774500.014.592584120
TCGGAAG763200.014.553721118-119
ATCGGAA900950.012.457656118-119
GATCGGA893400.012.454425116-117
AGATCGG995200.011.223861116-117
TAGATCG238600.010.837152118-119
CTAGATC62200.010.365624116-117
TTAGATC101300.09.204292120
AAGATCG419600.09.179488114-115
CACACGT37250.08.581965120
GAGATCG358400.08.510777120
TCTAGAT33050.08.245681116-117
CAGATCG318250.07.623223116-117
CGCTAGA8952.6815542E-87.426012108-109
Produced by FastQC (version 0.11.5)