FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12280902
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA1757500.014.8233185120
CGTATCA721200.08.985019120
GTATCAT703250.08.617129120
TCATTAA567550.08.58426120
CCGTATC795950.08.563347120
CGCCGTA888000.08.36486120
GCCGTAT847550.08.226057120
GGTCGCC1072450.08.212965120
GTCGCCG1037900.08.185754120
ATCATTA639700.08.085034120
TATCATT728100.08.018124120
GTGGTCG1213750.07.8401594120
GGTGGTC1304600.07.758695120
TGGTCGC1133050.07.7578163120
CATTAAA544000.07.7205825120
TCGCCGT972350.07.6700726120
CCCCCCC12600.07.61904344-45
CGGTGGT1415550.07.379459120
ATTAAAA633400.07.1234555120
GCGTCGG73600.07.0923867120
Produced by FastQC (version 0.11.5)