FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13451933
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA2527350.014.499779120
TCTCGGG25950.012.257686120
GCGTCGG73400.010.793141120
CGTATCA993100.09.022706120
GTATCAT975450.08.767582120
TCATTAA814150.08.705935120
ATCATTA901750.08.452546120
CATTAAA793050.08.430526120
CCGTATC1098050.08.072878120
GCCGTAT1164200.08.067829120
TATCATT1000650.07.8090606120
CGCCGTA1237600.07.7978654120
GTCGCCG1417800.07.733811120
TCGCCGT1347400.07.705832120
GGTCGCC1477250.07.520079120
GTGGTCG1664950.07.375211120
TGGTCGC1560500.07.365038120
GGTGGTC1775600.07.0271516120
ATTAAAA938150.06.9538813120
CGGTGGT1901300.06.5389547118-119
Produced by FastQC (version 0.11.5)