FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13451933
Sequences flagged as poor quality0
Sequence length50-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC174450.0100.23243120
GAGCACA285500.064.21215120
AGAGCAC394150.045.650475118-119
AAGAGCA602600.030.026175118-119
GAAGAGC655400.027.485659116-117
GGAAGAG817700.022.137897116-117
CGGAAGA960650.018.959116114-115
TCGGAAG972350.018.51534114-115
ATCGGAA1148650.015.845822112-113
GATCGGA1187750.015.190013112-113
AGATCGG1272400.014.265152110-111
TAGATCG291150.013.439332110-111
CTAGATC58250.012.455551112-113
CACACGT39650.012.267553120
GAGATCG497250.011.445053110-111
CACGTCT45550.011.230902120
CAGATCG442050.010.7190275118-119
TTAGATC102750.010.610473120
AAGATCG393700.010.533658116-117
ACACGTC42650.010.4214945120
Produced by FastQC (version 0.11.5)