FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11694873
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCGC286450.08.776708120
AGATCTC549650.08.722219120
TCGGTGG404950.08.697675120
GGTGGTC343250.08.582967120
GTGGTCG318250.08.503047120
TAGATCT562050.08.3162775118-119
GCGTCGG101650.08.263939120
GATCTCG560600.08.262865120
AGTGTAG749400.08.246886120
GCCTAGA11706.253249E-78.205416120
GGTCGCC293400.08.200696120
ATCTCGG501050.08.191086120
GAGTGTA799100.07.8616195120
TGTAGAT732350.07.832595120
CGTATCA187350.07.8145247120
GTGTAGA726300.07.7408743120
AGAGTGT857050.07.6730995120
CGGTGGT392700.07.639687120
TCTCGGT450700.07.6284003120
CTCGGTG439850.07.584669120
Produced by FastQC (version 0.11.5)