FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11694873
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC136850.080.70543120
GAGCACA221300.049.9384120
AGAGCAC298800.037.465878120
AAGAGCA448650.025.211004120
GAAGAGC522900.021.774815120
GGAAGAG624050.018.366045118-119
CGGAAGA751850.015.534684120
TCGGAAG758950.015.389356120
ATCGGAA882800.013.408301120
GATCGGA914850.013.229741120
AGATCGG1020600.011.768736118-119
TAGATCG234450.011.507589120
CTAGATC53050.010.81592116-117
TTAGATC80200.09.241314114-115
GAGATCG415400.09.142213118-119
CGCTAGA9205.456968E-128.909154118-119
GCTAGAT23000.08.90904120
AGAGATC141900.08.712318120
AAGATCG343550.08.668743110-111
CAGATCG370450.08.380575116-117
Produced by FastQC (version 0.11.5)