FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20167728
Sequences flagged as poor quality0
Sequence length52-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA2237100.012.859534120
CGTATCA926900.09.644404120
GCCGTAT1130800.09.162808120
GTATCAT927650.09.138607120
CCGTATC1063600.09.030983120
TCATTAA784800.08.883194120
ATCATTA878900.08.8809595120
CATTAAA753400.08.64821120
CGCCGTA1223750.08.515872120
GGTCGCC1492450.08.289167120
TGGTCGC1557950.08.275703120
TATCATT1025950.08.186982120
GTCGCCG1470600.08.138987120
TCGCCGT1370400.08.077391120
GCGTCGG165250.07.769523120
GTGGTCG1694050.07.7347884120
GGTGGTC1811900.07.360838120
TCGGTGG2052350.07.1854253120
CGGTGGT1943800.07.092845120
CTCGGTG2192700.06.72003120